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Hygromycin B EvoPure®
Product overview
HPLC Analysis of Hygromycin B from different suppliers
HPLC Major Impurities (Over 1%) for different supplier's Hygromycin B Identified as different rention times
| HPLC Retention Time | Batch of Hygromycin B | Impurities of Hygromycin B | ||||
|---|---|---|---|---|---|---|
| Supplier A (%) | Supplier B (%) | Supplier C (%) | TOKU-E(%) | EvoPure®(%) | ||
| 6.0 min | 2.17 | -- | 2.27 | -- | -- | A |
| 6.9 min | 5.11 | 1.0 | 5.97 | -- | -- | C |
| 7.1 min | 1.59 | -- | -- | -- | -- | D |
| 7.4 min | 6.54 | 1.75 | 5.92 | 1.2 | 0.5 | E |
| 8.1 min | 18.5 | 14.35 | 15.4 | 1.55 | -- | F |
| 8.6 min | 64.9 | 79.4 | 66.7 | 94.7 | 99.0 | Hygromycin B |
Survival rate of resistant cells at high concentrations hygromycin B (300 μg/mL)
| Supplier A | Supplier B | TOKU-E normal Hygromycin | EvoPure® | |
|---|---|---|---|---|
| Tobacco Calli cells transformed with the Hygromycin B phosphotransferase gene |
2 | 6 | 86 | 100 |
| 293 cells transformed with the Hygromycin B phosphotransferase gene |
1 | 8 | 91 | 100 |